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α-Phellandrene attenuates tissular damage, oxidative stress, and TNF-α levels on acute model ifosfamide-induced hemorrhagic cystitis in mice.
Gonçalves, R L G; Cunha, F V M; Sousa-Neto, B P S; Oliveira, L S A; Lopes, M E; Rezende, D C; Sousa, I J O; Nogueira, K M; Souza, L K M; Medeiros, J V R; Wong, D V T; Pereira, V M P; Lima-Júnior, R C P; Sousa, D P; Oliveira, C P C; Almeida, F R C; Oliveira, Francisco de Assis.
Affiliation
  • Gonçalves RLG; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Cunha FVM; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Sousa-Neto BPS; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Oliveira LSA; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Lopes ME; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Rezende DC; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Sousa IJO; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Nogueira KM; Experimental Physiopharmacology of Gastrointestinal Disorders, Federal University of Piauí, Av. São Sebastião, no 2819, Parnaíba, Piauí, 64202-020, Brazil.
  • Souza LKM; Experimental Physiopharmacology of Gastrointestinal Disorders, Federal University of Piauí, Av. São Sebastião, no 2819, Parnaíba, Piauí, 64202-020, Brazil.
  • Medeiros JVR; Experimental Physiopharmacology of Gastrointestinal Disorders, Federal University of Piauí, Av. São Sebastião, no 2819, Parnaíba, Piauí, 64202-020, Brazil.
  • Wong DVT; Department of Physiology and Pharmacology, Faculty of Medicine, Universidade Federal do Ceará, Rua Cel. Nunes de Melo, 1127, Fortaleza, Ceará, 60430-270, Brazil.
  • Pereira VMP; Department of Physiology and Pharmacology, Faculty of Medicine, Universidade Federal do Ceará, Rua Cel. Nunes de Melo, 1127, Fortaleza, Ceará, 60430-270, Brazil.
  • Lima-Júnior RCP; Department of Physiology and Pharmacology, Faculty of Medicine, Universidade Federal do Ceará, Rua Cel. Nunes de Melo, 1127, Fortaleza, Ceará, 60430-270, Brazil.
  • Sousa DP; Department of Pharmaceutical Sciences, Federal University of Paraíba, Centro de Ciências da Saúde, João Pessoa, Paraíba, 58059-900, Brazil.
  • Oliveira CPC; Department of Community Medicine, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Almeida FRC; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil.
  • Oliveira FA; Medicinal Plants Research Center, Federal University of Piauí, Av. Nossa Senhora de Fátima s/n, Teresina, PI, 64049-550, Brazil. fassisol@ufpi.edu.br.
Naunyn Schmiedebergs Arch Pharmacol ; 393(10): 1835-1848, 2020 10.
Article de En | MEDLINE | ID: mdl-32415495
ABSTRACT
Hemorrhagic cystitis (HC) is the major dose-limiting adverse effect of the clinical use ifosfamide (IFOS). The incidence of this side effect can be as high as 75%. Mesna has been used to reduce the risk of HC, although 5% of patients who get IFOS treatment may still suffer from HC. In previous studies, our group demonstrated that α-phellandrene (α-PHE) possesses anti-inflammatory activity, which opens the door for its study in the attenuation of HC. The objective of this study was to investigate the potential uroprotective effect of the α-PHE in the mouse model of IFOS-induced HC. In order to analyze the reduction of the urothelial damage, the bladder wet weight, hemoglobin content, and the Evans blue dye extravasation from the bladder matrix were evaluated. To investigate the involvement of neutrophil migration and lipid peroxidation and involvement of enzymatic and endogenous non-enzymatic antioxidants, the tissue markers myeloperoxidase (MPO), malondialdehyde, nitrite/nitrate (NOx), superoxide dismutase (SOD), and reduced glutathione (GSH) were evaluated. TNF-α and IL-1ß were measured by ELISA immunoassay technique. The results show that pretreatment with α-PHE significantly reduced urothelial damage that was accompanied by a decrease in the activity of MPO, MDA, and NOx levels and prevention of the depletion of SOD and GSH in bladder tissues. In the assessment of cytokines, α-PHE was able to significantly reduce TNF-α level. However, it does not affect the activities of IL-1ß. These data confirm that α-PHE exerts potent anti-inflammatory properties and demonstrates that α-PHE represents a promising therapeutic option for this pathological condition.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Facteur de nécrose tumorale alpha / Stress oxydatif / Cystite / Cyclohexane monoterpenes / Hémorragie / Ifosfamide Limites: Animals Langue: En Journal: Naunyn Schmiedebergs Arch Pharmacol Année: 2020 Type de document: Article Pays d'affiliation: Brésil

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Facteur de nécrose tumorale alpha / Stress oxydatif / Cystite / Cyclohexane monoterpenes / Hémorragie / Ifosfamide Limites: Animals Langue: En Journal: Naunyn Schmiedebergs Arch Pharmacol Année: 2020 Type de document: Article Pays d'affiliation: Brésil
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