Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests.
PLoS Negl Trop Dis
; 14(6): e0008364, 2020 06.
Article
de En
| MEDLINE
| ID: mdl-32492018
ABSTRACT
Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) is one of the most widespread medically important arboviruses, causing human infections that result in mortality rates of up to 60%. We describe the selection of a high-affinity small protein (Affimer-NP) that binds specifically to the nucleoprotein (NP) of CCHFV. We demonstrate the interference of Affimer-NP in the RNA-binding function of CCHFV NP using fluorescence anisotropy, and its inhibitory effects on CCHFV gene expression in mammalian cells using a mini-genome system. Solution of the crystallographic structure of the complex formed by these two molecules at 2.84 Å resolution revealed the structural basis for this interference, with the Affimer-NP binding site positioned at the critical NP oligomerization interface. Finally, we validate the in vitro application of Affimer-NP for the development of enzyme-linked immunosorbent and lateral flow assays, presenting the first published point-of-care format test able to detect recombinant CCHFV NP in spiked human and animal sera.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Réplication virale
/
Virus de la fièvre hémorragique de Crimée-Congo
/
Colorimétrie
/
Tests diagnostiques courants
/
Fièvre hémorragique de Crimée-Congo
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
PLoS Negl Trop Dis
Sujet du journal:
MEDICINA TROPICAL
Année:
2020
Type de document:
Article
Pays d'affiliation:
Royaume-Uni