Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry.
Methods Mol Biol
; 2163: 281-291, 2020.
Article
de En
| MEDLINE
| ID: mdl-32766984
ABSTRACT
Flow cytometry is one of the most widely used techniques for the detection of surface markers on various cells, particularly the cells of the immune system, at a single-cell resolution. Modern flow cytometers can identify rare cell population in highly heterogeneous samples. Here we present a protocol that allows a precise detection of basophils as well as eosinophils and neutrophils in induced sputum samples. The identification of sputum basophils and other granulocytes contributes to a better understanding of the cellular network that promotes and regulates inflammation of the lower respiratory tract.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Expectoration
/
Granulocytes basophiles
/
Immunophénotypage
/
Cytométrie en flux
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
Methods Mol Biol
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2020
Type de document:
Article
Pays d'affiliation:
Suisse