Real-Time Intracellular Temperature Imaging Using Lanthanide-Bearing Polymeric Micelles.
Nano Lett
; 20(9): 6466-6472, 2020 09 09.
Article
de En
| MEDLINE
| ID: mdl-32787172
ABSTRACT
Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to â¼20 degrees and subcellular gradients of â¼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lanthanides
Langue:
En
Journal:
Nano Lett
Année:
2020
Type de document:
Article
Pays d'affiliation:
Espagne