Effect of vitamin D supplementation on N-glycan branching and cellular immunophenotypes in MS.
Ann Clin Transl Neurol
; 7(9): 1628-1641, 2020 09.
Article
de En
| MEDLINE
| ID: mdl-32830462
ABSTRACT
OBJECTIVE:
To investigate the effect of cholecalciferol (vitamin D3) supplementation on peripheral immune cell frequency and N-glycan branching in patients with relapsing-remitting multiple sclerosis (RRMS).METHODS:
Exploratory analysis of high-dose (20 400 IU) and low-dose (400 IU) vitamin D3 supplementation taken every other day of an 18-month randomized controlled clinical trial including 38 RRMS patients on stable immunomodulatory therapy (NCT01440062). We investigated cholecalciferol treatment effects on N-glycan branching using L-PHA stain (phaseolus vulgaris leukoagglutinin) at 6 months and frequencies of T-, B-, and NK-cell subpopulations at 12 months with flow cytometry.RESULTS:
High-dose supplementation did not change CD3+ T cell subsets, CD19+ B cells subsets, and NK cells frequencies, except for CD8+ T regulatory cells, which were reduced in the low-dose arm compared to the high-dose arm at 12 months. High-dose supplementation decreased N-glycan branching on T and NK cells, measured as L-PHA mean fluorescence intensity (MFI). A reduction of N-glycan branching in B cells was not significant. In contrast, low-dose supplementation did not affect N-glycan branching. Changes in N-glycan branching did not correlate with cell frequencies.INTERPRETATION:
Immunomodulatory effect of vitamin D may involve regulation of N-glycan branching in vivo. Vitamin D3 supplementation did at large not affect the frequencies of peripheral immune cells.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Polyosides
/
Cellules tueuses naturelles
/
Sous-populations de lymphocytes B
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Sous-populations de lymphocytes T
/
Cholécalciférol
/
Compléments alimentaires
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Sclérose en plaques récurrente-rémittente
/
Facteurs immunologiques
Type d'étude:
Clinical_trials
Limites:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Langue:
En
Journal:
Ann Clin Transl Neurol
Année:
2020
Type de document:
Article
Pays d'affiliation:
Allemagne