An enzyme-triggered turn-on fluorescent probe based on carboxylate-induced detachment of a fluorescence quencher.
Org Biomol Chem
; 18(42): 8620-8624, 2020 11 04.
Article
de En
| MEDLINE
| ID: mdl-32832959
ABSTRACT
We developed a new class of turn-on fluorescent probes for an esterase. After the esterase-mediated hydrolysis produced carboxylate (as a fluorescence activator), the fluorescence intensity was markedly increased through the detachment of a quencher moiety from the quenched Cy5 fluorophore. Because the probes based on this new activator-induced quencher-detachment (AiQd) adopt a non-immolative linker between the cleavable site and the fluorophore, the rate of the enzymatic reaction is greatly improved, without the generation of any by-products.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Colorants fluorescents
Langue:
En
Journal:
Org Biomol Chem
Sujet du journal:
BIOQUIMICA
/
QUIMICA
Année:
2020
Type de document:
Article