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DNA extraction from individual Phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) specimens: Which is the method with better results?
Leonel, João Augusto Franco; Vioti, Geovanna; Alves, Maria Luana; da Silva, Diogo Tiago; Meneghesso, Pedro Armando; Benassi, Julia Cristina; Spada, Julio Cesar Pereira; Galvis-Ovallos, Fredy; Soares, Rodrigo Martins; Oliveira, Trícia Maria Ferreira de Sousa.
Affiliation
  • Leonel JAF; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
  • Vioti G; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
  • Alves ML; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
  • da Silva DT; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
  • Meneghesso PA; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil.
  • Benassi JC; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil.
  • Spada JCP; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
  • Galvis-Ovallos F; Laboratory of Entomology in Public Health, School of Public Health, University of São Paulo - USP, São Paulo, São Paulo state, Brazil.
  • Soares RM; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Department of Preventive Veterinary Medicin
  • Oliveira TMFS; Laboratory of Applied Preventive Veterinary Medicine, Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo - USP, Pirassununga, São Paulo state, Brazil; Post-graduate Program in Experimental Epidemiology Applied to Zoonoses, Department of Prevent
Exp Parasitol ; 218: 107981, 2020 Nov.
Article de En | MEDLINE | ID: mdl-32871144
ABSTRACT
Phlebotomine sand flies (Diptera Psychodidae Phlebotominae) are a group of small insects of great concern for Public Health. These dipterous are intensely studied worldwide due to their involvement in the transmission of several pathogens, mainly Leishmania spp. parasites. Nowadays, the molecular tools have been included in Phlebotomine sand flies studies and has shown to be powerful tools in bioecology studies of these dipterous. Thereby, when molecular approaches are employed, there is a great concern regarding the amount and quality of the DNA obtained for analysis. Here, seven methods of DNA extraction, between commercial kits and in house extraction protocols were evaluated. We considered measure of DNA concentration and purity ratios using a spectrophotometer to check the performance of each protocol. In addition, the quality evaluation of the DNA extracted was performed by endogenous gene PCR on samples. The results of the seven evaluated DNA extraction protocols and their implications are discussed.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Psychodidae / ADN Type d'étude: Health_economic_evaluation Limites: Animals Langue: En Journal: Exp Parasitol Année: 2020 Type de document: Article
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Psychodidae / ADN Type d'étude: Health_economic_evaluation Limites: Animals Langue: En Journal: Exp Parasitol Année: 2020 Type de document: Article