The larval haematopoietic organs of Manduca sexta (Insecta, Lepidoptera): An insight into plasmatocyte development and larval haematopoiesis.
Dev Comp Immunol
; 115: 103858, 2021 02.
Article
de En
| MEDLINE
| ID: mdl-32898576
ABSTRACT
Haematopoietic organs (HOs) in Lepidoptera are widely recognised as the source for at least two haemocyte types. With new specific markers for oenocytoids and spherule cells and a method to identify prohaemocytes, the haemocytes formed in and released by the HOs of Manduca sexta are characterised. Differentiation of HO-cells to haemocytes other than plasmatocytes and prohaemocytes neither occurs in the organ itself nor in cells released in vitro by the HOs. Differential labelling patterns evidence the existence of plasmatocyte subpopulations and prohaemocytes, which might represent a gradual differentiation of haemocytes within the organs. Prohaemocytes can be identified by PNA-labelling of the cell membrane. These prohaemocytes are found in circulation and in the HOs and are released by the organs. Circulating prohaemocytes possess characteristics for granular cells, plasmatocytes or oenocytoids while HO derived prohaemocytes share characteristics only with plasmatocytes. Ablation of the HOs diminishes the plasmatocyte and prohaemocyte number, indicating a true larval haematopoietic function.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Manduca
/
Hématopoïèse
/
Hémocytes
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Dev Comp Immunol
Année:
2021
Type de document:
Article