Development of an antigen-capture enzyme-linked immunosorbent assay for diagnosis of Aleutian mink disease virus.
Arch Virol
; 166(1): 83-90, 2021 Jan.
Article
de En
| MEDLINE
| ID: mdl-33068192
ABSTRACT
Aleutian mink disease (AMD), caused by Aleutian mink disease virus (AMDV), is a very important infectious disease of mink. Currently, elimination of antibody- or antigen-positive animals is the most successful strategy for eradicating AMD, but the claw-cutting method of blood sampling is difficult to perform and painful for the animal. In this study, we aimed to establish an antigen capture enzyme-linked immunosorbent assay (AC-ELISA) method for the efficient detection of AMDV antigens using fecal samples. A purified mouse monoclonal antibody (mAb) was used as the capture antibody, and a rabbit polyclonal antibody (pAb) was used as the detection antibody. The assay was optimized by adjusting a series of parameters. Using a cutoff value of 0.205, the limit of detection of the AC-ELISA for strain AMDV-G antigen was 2 µg/mL, and there was no cross-reaction with other mink viruses. The intra- and inter-assay standard deviations were below 0.046, and the correlation of variance (CV) values were 1.24-7.12% when testing fecal samples. Compared with conventional PCR results, the specificity and sensitivity were 91.5% and 90.6%, respectively, and the concordance rate between the two methods was 91.1%.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Test ELISA
/
Virus de la maladie aléoutienne du vison
/
Maladie aléoutienne du vison
/
Visons
/
Antigènes
Type d'étude:
Diagnostic_studies
Limites:
Animals
Langue:
En
Journal:
Arch Virol
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine