Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR.
Mol Ecol Resour
; 21(5): 1452-1459, 2021 Jul.
Article
de En
| MEDLINE
| ID: mdl-33232563
ABSTRACT
The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Batrachochytrium (genre)
/
Amphibiens
/
Mycoses
Type d'étude:
Prognostic_studies
Limites:
Animals
Pays/Région comme sujet:
Africa
Langue:
En
Journal:
Mol Ecol Resour
Année:
2021
Type de document:
Article
Pays d'affiliation:
Royaume-Uni