A Novel Optimization of Water-Soluble Compound Polysaccharides from Chinese Herbal Medicines by Quantitative Theory and Study on Its Characterization and Antioxidant Activities.
Chem Biodivers
; 18(1): e2000688, 2021 Jan.
Article
de En
| MEDLINE
| ID: mdl-33258537
ABSTRACT
The present study optimized the extraction characterization and antioxidant activities of water-soluble compound polysaccharides (CPs) from hawthorn, lotus leaf, Fagopyrum tataricum, semen cassiae, Lycium barbarum, and Poria cocos Chinese herbal medicines that have mass ratios of 4 2 2 1.5 1 1. The CPs yield equation was predicted using quantitative theory, to which a maximum CPs yield of 7.18±0.24 % under the following optimal extraction conditions a water-to-raw material ratio of 30â
mL/g, an extraction temperature of 65 °C, an extraction time of 45â
min, and extraction mode ultrasonic-assistant extraction. CPs were consisted of Ara, Gal, Glc, Xyl, Man, GalA and GlcA in a molar ratio of 3.1 2.6 50.6 1.7 20.4 17.2 4.2. The HPGPC profiles and FT-IR spectra implied that CPs were heterogeneous acidic polysaccharides and possessed the ß-d-pyranose configuration. Congo red test, CD spectrum and SEM revealed that CPs with three helix conformation showed a flocculent, granulous or sheet-like appearance. Furthermore, the relationships between antioxidant activity and concentration of CPs displayed significant positive correlation, and the scavenging abilities for DPPH, hydroxyl radical, ABTS, superoxide-anion radical and reducing power of CPs were 93.56±2.51 %, 84.03±1.69 %, 83.29±1.93 %, 37.49±1.93 % and 0.467±0.006 at a concentration of 4.0â
mg/mL. Therefore, CPs could be applied as a potential natural antioxidant in pharmaceutical or functional food fields.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Polyosides
/
Médicaments issus de plantes chinoises
/
Antioxydants
Langue:
En
Journal:
Chem Biodivers
Sujet du journal:
BIOQUIMICA
/
QUIMICA
Année:
2021
Type de document:
Article