Intracellular role of IL-6 in mesenchymal stromal cell immunosuppression and proliferation.
Sci Rep
; 10(1): 21853, 2020 12 14.
Article
de En
| MEDLINE
| ID: mdl-33318571
ABSTRACT
Interleukin (IL)-6 is a pleiotropic cytokine involved in the regulation of hematological and immune responses. IL-6 is secreted chiefly by stromal cells, but little is known about its precise role in the homeostasis of human mesenchymal stromal cells (hMSCs) and the role it may play in hMSC-mediated immunoregulation. We studied the role of IL-6 in the biology of bone marrow derived hMSC in vitro by silencing its expression using short hairpin RNA targeting. Our results show that IL-6 is involved in immunosuppression triggered by hMSCs. Cells silenced for IL-6 showed a reduced capacity to suppress activated T-cell proliferation. Moreover, silencing of IL-6 significantly blocked the capacity of hMSCs to proliferate. Notably, increasing the intracellular level of IL-6 but not recovering the extracellular level could restore the proliferative impairment observed in IL-6-silenced hMSC. Our data indicate that IL-6 signals in hMSCs by a previously undescribed intracellular mechanism.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes T
/
Transduction du signal
/
Interleukine-6
/
Prolifération cellulaire
/
Cellules souches mésenchymateuses
/
Tolérance immunitaire
Limites:
Humans
Langue:
En
Journal:
Sci Rep
Année:
2020
Type de document:
Article
Pays d'affiliation:
Espagne