The Acute Phase Protein Hepcidin Is Cytotoxic to Human and Mouse Myeloma Cells.
Anticancer Res
; 41(2): 601-608, 2021 Feb.
Article
de En
| MEDLINE
| ID: mdl-33517264
ABSTRACT
BACKGROUND/AIM:
Hepcidin is a cationic acute phase reactant synthesized by the liver. It has bactericidal properties and is a major regulator of iron homeostasis. Cationic antimicrobial peptides represent an innate antimicrobial defense system. We hypothesized that, like other cationic antimicrobial peptides, hepcidin is cytotoxic to cancer cells. MATERIALS ANDMETHODS:
The cytotoxicity of human hepcidin against myeloma cells was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. Plasma membrane damage was quantified by propidium iodide (PI) staining. Cell membrane changes were visualized by scanning electron microscopy.RESULTS:
Hepcidin impaired myeloma cell survival and induced DNA fragmentation. PI staining and scanning electron microscopy revealed hepcidin-induced disruption of the plasma membrane.CONCLUSION:
Human hepcidin is an anti-cancer peptide that induces myeloma cell lysis, and therefore may play a role in innate anticancer immunity. To our knowledge, this is the first biological function ascribed to human hepcidin that is not related to its antimicrobial and iron-regulatory properties.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Fragments peptidiques
/
Hepcidines
/
Myélome multiple
/
Antinéoplasiques
Limites:
Animals
/
Humans
Langue:
En
Journal:
Anticancer Res
Année:
2021
Type de document:
Article
Pays d'affiliation:
Canada