Kaempferol suppresses proliferation and induces apoptosis and DNA damage in human gallbladder cancer cells through the CDK4/CDK6/cyclin D1 pathway.
Eur Rev Med Pharmacol Sci
; 25(3): 1311-1321, 2021 02.
Article
de En
| MEDLINE
| ID: mdl-33629301
ABSTRACT
OBJECTIVE:
Kaempferol has been reported to play an anti-tumor role in various human cancers, while its role in gallbladder cancer (GBC) is unclear. MATERIALS ANDMETHODS:
We found that kaempferol significantly inhibited the growth, invasion and migration, meanwhile induced apoptosis through cells arrested at G0/G1 phase of GBC cell lines, including GBC-SD and SGC996 cells in vitro.RESULTS:
Kaempferol promoted the release of cytochrome C from the mitochondria to cytoplasm, the activation of c-caspase-3 and c-caspase-9 and increased the expression levels of pro-apoptotic factor Bax, meanwhile decreased the expression levels of anti-apoptotic factor Bcl-2. In addition, the expression levels of CDK4, CDK6 and cyclin D1, which are members of the CDK4/CDK6/cyclin D1 signaling pathway, were also decreased by kaempferol. Moreover, kaempferol could efficiently prevent tumor progression of GBC in the xenograft in vivo.CONCLUSIONS:
Our results demonstrated that kaempferol suppressed GBC progression through activation of the CDK4/CDK6/cyclin D1 signaling pathway, suggesting that it might be a potential anti-tumor agent for clinical treatment of GBC.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Apoptose
/
Cycline D1
/
Kaempférols
/
Kinase-4 cycline-dépendante
/
Kinase-6 cycline-dépendante
/
Tumeurs de la vésicule biliaire
/
Antinéoplasiques
Limites:
Animals
/
Female
/
Humans
Langue:
En
Journal:
Eur Rev Med Pharmacol Sci
Sujet du journal:
FARMACOLOGIA
/
TOXICOLOGIA
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine