H2S improves doxorubicin-induced myocardial fibrosis by inhibiting oxidative stress and apoptosis via Keap1-Nrf2.
Technol Health Care
; 29(S1): 195-209, 2021.
Article
de En
| MEDLINE
| ID: mdl-33682759
ABSTRACT
OBJECTIVE:
We waimed to investigate whether H2S can relieve the myocardial fibrosis caused by doxorubicin through Keap1-Nrf2.METHODS:
Sprague-Dawley (SD) rats were randomly divided into four groups normal control group (Control); DOX model group (DOX); H2S intervention model group (DOX+H2S); H2S control group (H2S). DOX and DOX+H2S group were injected with doxorubicin (3.0 mg/kg/time) intraperitoneally. Both of the Control group and H2S groups were given normal saline in equal volume, 2 weeks later, DOX+H2S and H2S group were controlled with NaHS (56 µmol/kg/d) through the abdominal cavity, while the Control and DOX group were injected with normal saline of the same dosage intraperitoneally.RESULTS:
Myocardial injury and myocardial cell apoptosis were significantly increased, the H2S content in myocardial tissue was remarkably down-regulated, the expression levels of MDA, Keap1, caspase-3, caspase-9, TNF-α, IL1ß, MMPs and TIMP-1 in rat myocardial tissue was significantly up-regulated (P< 0.05), and the expression levels of GSH, NQO1, Bcl-2 were down-regulated compared with those of control group. The above results can be reversed by the DOX+H2S group. There is no statistically significant difference between the Control group and the H2S control group.CONCLUSIONS:
These results suggest that H2S can improve DOX-induced myocardial fibrosis in rats, and the keap1/Nrf2 signaling pathway, oxidative stress, inflammation, and apoptosis may be involved in the mechanism.Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Doxorubicine
/
Facteur-2 apparenté à NF-E2
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Technol Health Care
Sujet du journal:
ENGENHARIA BIOMEDICA
/
SERVICOS DE SAUDE
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine