Tracking the fate and migration of cells in live animals with cell-cycle indicators and photoconvertible proteins.
J Neurosci Methods
; 355: 109127, 2021 05 01.
Article
de En
| MEDLINE
| ID: mdl-33722643
ABSTRACT
Cell migration and cell proliferation are the basic principles that make up a living organism, and both biologically and medically. In order to understand living organism and biological phenomena, it is essential to track the migration, proliferation, and fate of cells in living cells and animals and to clarify the properties and molecular expression of cells. Recent developments in novel fluorescent proteins have made it possible to observe cell migration and proliferation as the cell cycle at the single-cell level in living individuals and tissues. Here, we introduce cell cycle visualization of living cells and animals by Fucci (Fluorescent Ubiquitination-based Cell Cycle Indicator) system and in situ cell labeling of cells and tracking cell migration by photoactivatable and photoconvertible proteins. In addition, we will present our established methods as an example of combines above tools with single-cell molecular expression analysis to reveal the fate of migrating cells at single cell level.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Protéines luminescentes
Limites:
Animals
Langue:
En
Journal:
J Neurosci Methods
Année:
2021
Type de document:
Article