A rapid and accurate method for screening T-2 toxin in food and feed using competitive AlphaLISA.
FEMS Microbiol Lett
; 368(6)2021 04 22.
Article
de En
| MEDLINE
| ID: mdl-33755724
ABSTRACT
T-2 is a common mycotoxin contaminating cereal crops. Chronic consumption of food contaminated with T-2 toxin can lead to death, so simple and accurate detection methods in food and feed are necessary. In this paper, we establish a highly sensitive and accurate method for detecting T-2 toxin using AlphaLISA. The system consists of acceptor beads labeled with T-2-bovine serum albumin (BSA), streptavidin-labeled donor beads and biotinylated T-2 antibodies. T-2 in the sample matrix competes with T-2-BSA for antibodies. Adding biotinylated antibodies to the test well followed by T-2 and T-2-BSA acceptor beads yielded a detection range of 0.03-500 ng/mL. The half-maximal inhibitory concentration was 2.28 ng/mL and the coefficient of variation was <10%. In addition, this method had no cross-reaction with other related mycotoxins. This optimized method for extracting T-2 from food and feed samples achieved a recovery rate of approximately 90% in T-2 concentrations as low as 1 ng/mL, better than the performance of a commercial ELISA kit. This competitive AlphaLISA method offers high sensitivity, good specificity, good repeatability and simple operation for detecting T-2 toxin in food and feed.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Contamination des aliments
/
Analyse d'aliment
/
Aliment pour animaux
Type d'étude:
Diagnostic_studies
/
Screening_studies
Langue:
En
Journal:
FEMS Microbiol Lett
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine