Burkholderia thailandensis E264 as a promising safe rhamnolipids' producer towards a sustainable valorization of grape marcs and olive mill pomace.
Appl Microbiol Biotechnol
; 105(9): 3825-3842, 2021 May.
Article
de En
| MEDLINE
| ID: mdl-33880598
ABSTRACT
Within the circular economy framework, our study aims to assess the rhamnolipid production from winery and olive oil residues as low-cost carbon sources by nonpathogenic strains. After evaluating various agricultural residues from those two sectors, Burkholderia thailandensis E264 was found to use the raw soluble fraction of nonfermented (white) grape marcs (NF), as the sole carbon and energy source, and simultaneously, reducing the surface tension to around 35 mN/m. Interestingly, this strain showed a rhamnolipid production up to 1070 mg/L (13.37 mg/g of NF), with a higher purity, on those grape marcs, predominately Rha-Rha C14-C14, in MSM medium. On olive oil residues, the rhamnolipid yield of using olive mill pomace (OMP) at 2% (w/v) was around 300 mg/L (15 mg/g of OMP) with a similar CMC of 500 mg/L. To the best of our knowledge, our study indicated for the first time that a nonpathogenic bacterium is able to produce long-chain rhamnolipids in MSM medium supplemented with winery residues, as sole carbon and energy source. KEY POINTS ⢠Winery and olive oil residues are used for producing long-chain rhamnolipids (RLs). ⢠Both higher RL yields and purity were obtained on nonfermented grape marcs as substrates. ⢠Long-chain RLs revealed stabilities over a wide range of pH, temperatures, and salinities.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Vitis
/
Olea
/
Minorités sexuelles
Limites:
Humans
/
Male
Langue:
En
Journal:
Appl Microbiol Biotechnol
Année:
2021
Type de document:
Article
Pays d'affiliation:
Italie