Evaluation of Cellular and Serological Responses to Acute SARS-CoV-2 Infection Demonstrates the Functional Importance of the Receptor-Binding Domain.
J Immunol
; 206(11): 2605-2613, 2021 06 01.
Article
de En
| MEDLINE
| ID: mdl-33952616
ABSTRACT
The factors that control the development of an effective immune response to the recently emerged SARS-CoV-2 virus are poorly understood. In this study, we provide a cross-sectional analysis of the dynamics of B cell responses to SARS-CoV-2 infection in hospitalized COVID-19 patients. We observe changes in B cell subsets consistent with a robust humoral immune response, including significant expansion of plasmablasts and activated receptor-binding domain (RBD)-specific memory B cell populations. We observe elevated titers of Abs to SARS-CoV-2 RBD, full-length Spike, and nucleoprotein over the course of infection, with higher levels of RBD-specific IgG correlating with increased serum neutralization. Depletion of RBD-specific Abs from serum removed a major portion of neutralizing activity in most individuals. Some donors did retain significant residual neutralization activity, suggesting a potential Ab subset targeting non-RBD epitopes. Taken together, these findings are instructive for future vaccine design and mAb strategies.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lymphocytes B
/
Protéines nucléocapside
/
Glycoprotéine de spicule des coronavirus
/
SARS-CoV-2
/
COVID-19
/
Immunité cellulaire
/
Mémoire immunologique
Type d'étude:
Evaluation_studies
Limites:
Female
/
Humans
/
Male
Langue:
En
Journal:
J Immunol
Année:
2021
Type de document:
Article
Pays d'affiliation:
Gabon