Liquid Storage of Geophagus brasiliensis semen in the Presence of Different Extenders.
Cryo Letters
; 41(4): 202-208, 2020.
Article
de En
| MEDLINE
| ID: mdl-33988648
ABSTRACT
BACKGROUND:
In order to preserve the genetic diversity of cichlid fish in gene banks, it is necessary to use certain extenders to maintain the integrity of spermatozoa cells during cooling.OBJECTIVE:
To evaluate the effects of different extenders on the quality parameters of cooled semen of Geophagus brasiliensis. MATERIALS ANDMETHODS:
Semen samples were collected from seven adult fish and diluted with five extenders Beltsville Thawing Solution (BTS™), Hanks' Balanced Salt Solution (HBSS), Tris-glucose, Ginsburg's Fish Ringers, and Phosphate buffered Saline. All parameters were evaluated in fresh semen samples and after cooling at 4°C at 0, 24, 48, and 96 h to evaluate cell viability (membrane integrity, DNA, and mitochondrial functionality) and motility rate and weather motility.RESULTS:
The BTS and Tris-glucose resulted in the best outcomes (P<0.05) in terms of membrane integrity assessments (35.1% and 30.9%, respectively), DNA integrity (71.6%; 75.7%), mitochondrial function (26.9%; 28.0%) and motility rate (8.6%; 8.6%), respectively, for semen cooled to 4°C for 96 h. However, the 48-h period motility after cooling in BTS was superior to all other treatments.CONCLUSION:
BTS and Tris-glucose can be considered as the best extenders for the cold storage of Geophagus brasiliensis spermatozoa.
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Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Conservation de semence
/
Cryoconservation
/
Cichlides
Limites:
Animals
Pays/Région comme sujet:
America do sul
/
Brasil
Langue:
En
Journal:
Cryo Letters
Sujet du journal:
BIOLOGIA
/
QUIMICA
Année:
2020
Type de document:
Article
Pays d'affiliation:
Brésil