Evaluation of the AGCU Expressmarker 30 Kit composed of 31 loci for forensic application.
Forensic Sci Int
; 324: 110849, 2021 Jul.
Article
de En
| MEDLINE
| ID: mdl-34030000
ABSTRACT
With the widespread use of STR in identification of individuals, paternity testing, as well as population genetics, many commercially robust and validated STR multiplex kits were developed. The AGCU Expressmarker 30 Kit is a new autosomal STR system that contains 29 autosomal STR loci (D3S1358, vWA, D1S1656, CSF1PO, D8S1132, D19S253, D3S3045, D8S1179, D21S11, D16S539, TPOX, D6S477, Penta D, D2S441, D5S818, TH01, FGA, D15S659, D22S1045, D19S433, D13S317, D7S820, D6S1043, D10S1435, D10S1248, D2S1338, D18S51, D12S391, and Penta E), one insertion/deletion polymorphic marker on the Y chromosome (Y indel), and the amelogenin locus. A series of validation studies were performed in this context according to the guidelines of "Validation Guidelines for Forensic DNA Analysis Methods". The sensitivity study showed that a full profile was observed with template DNA as low as 40 pg. In the stability study, all STR profiles were obtained at concentrations of humic acid up to 800 ng/µL, hematin up to 250 µM, and tannic acid up to 200 ng/µL. The mixture study demonstrated that all of the minor alleles could be called at ratios from 11-291 when the total DNA was 2 ng. In the population study, the total discrimination power for three population (Sichuan-Han, Gansu-Hui, and Guangxi-Zhuang) were above 0.9999999999999999999999999999999992, 0.999999999999999999999999999999998 and 0.999999999999999999999999999999994 as well as the cumulative probability of paternity exclusion were 0.999999999999953, 0.999999999999178, and 0.999999999999611 respectively. These results demonstrated that the AGCU Expressmarker 30 Kit is a useful tool for analyzing both forensic casework and database samples.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Profilage d'ADN
/
Répétitions microsatellites
/
Génétique légale
Type d'étude:
Evaluation_studies
Limites:
Animals
/
Humans
Langue:
En
Journal:
Forensic Sci Int
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine