Individual B cells transcribe multiple rearranged immunoglobulin light chains in teleost fish.
iScience
; 24(6): 102615, 2021 Jun 25.
Article
de En
| MEDLINE
| ID: mdl-34142062
ABSTRACT
B cells express a unique antibody protein which comprises two pairs of immunoglobulin (Ig) heavy (H) and light (L) chains. In addition to an invariable constant (C) region, IgH and IgL chains encompass a variable (V) region mediating antigen binding. This unique region stems from Ig V(D)J gene recombination, which generates diversity by assembling these gene segments into VHDJH and VLJL genes. To ensure that one B cell only expresses one antibody, VHDJH rearrangement occurs only in one IgH locus (allelic exclusion), whereas VLJL rearrangement only in either the κ or λ locus (isotype exclusion). However, teleosts express multiple IgLs encoded by distinct CL genes. Using single-cell transcriptomics, we have demonstrated the transcription of distinct rearranged VLJLCL genes in single rainbow trout B cells. Our results highlight the laxity of isotype exclusion in teleosts and strongly suggest that fish B cells can produce antibodies of different specificities.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Langue:
En
Journal:
IScience
Année:
2021
Type de document:
Article
Pays d'affiliation:
Espagne