Protocols for analysis of mitochondrial permeability transition pore opening in mouse somatic cell reprogramming.
STAR Protoc
; 2(2): 100568, 2021 06 18.
Article
de En
| MEDLINE
| ID: mdl-34151295
ABSTRACT
Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. Here, we describe a protocol for imaging mitochondrial permeability transition pore (mPTP) opening in reprogramming of somatic cells using a confocal microscope. We also describe a method to sort high and low mPTP opening somatic cells by calcein fluorescence and reprogram these sorted cells to iPSCs. These protocols are also suitable for imaging mPTP opening and uncovering the mechanisms of mPTP function in other cell fate conversions. For complete details on the use and execution of this protocol, please refer to Ying et al. (2018).
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Reprogrammation cellulaire
/
Pore de transition de perméabilité mitochondriale
Limites:
Animals
Langue:
En
Journal:
STAR Protoc
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine