An improved in vitro photochemical internalization protocol for 3D spheroid cultures.
Lasers Med Sci
; 36(8): 1567-1571, 2021 Oct.
Article
de En
| MEDLINE
| ID: mdl-34185168
ABSTRACT
Photochemical internalization (PCI) is a modified form of photodynamic therapy (PDT) that enhances the efficacy of therapeutic agents in a site and temporal specific manner in both in vitro and in vivo publications. The purpose of the study reported here was to evaluate the benefits of a modified PCI protocol in a 3D rat glioma spheroid model. In the modified protocol, F98 glioma cells were incubated with photosensitizer (AlPcS2a) prior to spheroid generation, as opposed to post-spheroid formation photosensitizer exposure commonly used in conventional protocols. The efficacy of both bleomycin and doxorubicin PCI was evaluated using either the conventional or modified protocols. The formed spheroids were then exposed to light treatment from a diode laser, λ= 670 nm. Spheroid growth was monitored for a period of 14 days. The results of spheroid growth assays showed that there was no statistically significant difference in PCI efficacy between the conventional and modified protocols for both of the drugs tested. The direct PDT effect was significantly reduced using the modified protocol. Therefore, due to its several advantages, the modified protocol is recommended for evaluating the efficacy of PCI in tumor spheroid models.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Photothérapie dynamique
/
Gliome
Type d'étude:
Guideline
Limites:
Animals
Langue:
En
Journal:
Lasers Med Sci
Sujet du journal:
BIOTECNOLOGIA
/
RADIOLOGIA
Année:
2021
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique