Chr15q25 Genetic Variant rs16969968 Alters Cell Differentiation in Respiratory Epithelia.
Int J Mol Sci
; 22(13)2021 Jun 22.
Article
de En
| MEDLINE
| ID: mdl-34206324
ABSTRACT
The gene cluster region, CHRNA3/CHRNA5/CHRNB4, encoding for nicotinic acetylcholine receptor (nAChR) subunits, contains several genetic variants linked to nicotine addiction and brain disorders. The CHRNA5 single-nucleotide polymorphism (SNP) rs16969968 is strongly associated with nicotine dependence and lung diseases. Using immunostaining studies on tissue sections and air-liquid interface airway epithelial cell cultures, in situ hybridisation, transcriptomic and cytokines detection, we analysed rs16969968 contribution to respiratory airway epithelial remodelling and modulation of inflammation. We provide cellular and molecular analyses which support the genetic association of this polymorphism with impaired ciliogenesis and the altered production of inflammatory mediators. This suggests its role in lung disease development.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Différenciation cellulaire
/
Régulation de l'expression des gènes
/
Récepteurs nicotiniques
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Muqueuse respiratoire
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Polymorphisme de nucléotide simple
/
Inflammation
/
Protéines de tissu nerveux
Limites:
Humans
Langue:
En
Journal:
Int J Mol Sci
Année:
2021
Type de document:
Article
Pays d'affiliation:
France