3D bioprinting of dual-crosslinked nanocellulose hydrogels for tissue engineering applications.
J Mater Chem B
; 9(31): 6163-6175, 2021 08 21.
Article
de En
| MEDLINE
| ID: mdl-34286810
ABSTRACT
Hydrogels based on cellulose nanofibrils (CNFs) have been widely used as scaffolds for biomedical applications, however, the poor mechanical properties of CNF hydrogels limit their use as ink for 3D bioprinting in order to generate scaffolds for tissue engineering applications. In this study, a dual crosslinkable hydrogel ink composed of a poly(ethylene glycol) (PEG) star polymer and 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO)-oxidized nanocellulose fibers (CNFs) is presented. As the resulting hydrogel had low structural integrity, at first crosslinking of CNFs was introduced by Ca2+. Strong physical interactions between CNFs and Ca2+ cations allowed easy regulation of the viscosity of the inks for extrusion printing raising the solution viscosity by more than 1.5 times depending on the amount of Ca2+ added. The resulting hydrogel had high structural integrity and was further stabilized in a second step by photo crosslinking of PEG under visible light. In only a few seconds, hydrogels with Young's modulus between â¼10 and 30 kPa were obtained just by altering the CNF and Ca2+ content. 3D printed hydrogels supported fibroblasts with excellent cell viability and proliferation. The dual crosslinkable hydrogel ink herein developed is versatile, easy to prepare, and suitable for 3D printing of bioscaffolds with highly tailored viscoelastic and mechanical properties applicable in a wide range of regenerative medicines.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Matériaux biocompatibles
/
Cellulose
/
Hydrogels
/
Réactifs réticulants
/
Ingénierie tissulaire
/
Impression tridimensionnelle
Limites:
Animals
Langue:
En
Journal:
J Mater Chem B
Année:
2021
Type de document:
Article
Pays d'affiliation:
Australie