Multiplexed direct detection of barcoded protein reporters on a nanopore array.
Nat Biotechnol
; 40(1): 42-46, 2022 01.
Article
de En
| MEDLINE
| ID: mdl-34385692
ABSTRACT
Detection of specific proteins using nanopores is currently challenging. To address this challenge, we developed a collection of over twenty nanopore-addressable protein tags engineered as reporters (NanoporeTERs, or NTERs). NTERs are constructed with a secretion tag, folded domain and a nanopore-targeting C-terminal tail in which arbitrary peptide barcodes can be encoded. We demonstrate simultaneous detection of up to nine NTERs expressed in bacterial or human cells using MinION nanopore sensor arrays.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Nanopores
Type d'étude:
Diagnostic_studies
Limites:
Humans
Langue:
En
Journal:
Nat Biotechnol
Sujet du journal:
BIOTECNOLOGIA
Année:
2022
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique