Discovery of Polycyclic Macrolide Shuangdaolides by Heterologous Expression of a Cryptic trans-AT PKS Gene Cluster.
Org Lett
; 23(17): 6967-6971, 2021 09 03.
Article
de En
| MEDLINE
| ID: mdl-34388000
ABSTRACT
A cryptic trans-acyltransferase polyketide synthase biosynthetic gene cluster sdl (80 kb) from Streptomyces sp. B59 was cloned and transferred into a heterologous host Streptomyces albus J1074, resulting in a class of polycyclic macrolide shuangdaolides A-D (1-4) and dumulmycin (5). Heterologous expression and gene inactivation experiments allowed the identification of two biosynthetic intermediates, 6 and 7, suggesting an unusual multidomain SDR oxidoreductase SdlR in charge of the formation of a rare 2-hydroxycyclopentenone moiety in this class of compounds.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Streptomyces
/
Inhibiteurs de la synthèse protéique
/
Macrolides
/
Polyketide synthases
/
Antibactériens
Langue:
En
Journal:
Org Lett
Sujet du journal:
BIOQUIMICA
Année:
2021
Type de document:
Article
Pays d'affiliation:
Chine