Sequential staining of HIV gp140 to capture antigen-specific human B cells via flow cytometry.
STAR Protoc
; 2(3): 100771, 2021 09 17.
Article
de En
| MEDLINE
| ID: mdl-34471908
ABSTRACT
Protocols for efficient capture of antigen-specific B cells (ASBCs) are useful for understanding pathogen-specific B-cell responses during natural infection or vaccination. Fluorescently labeled tetramerized probes are classically used to capture ASBCs, but many occlude valuable epitopes available for B-cell receptor binding. Here, we describe a bead assay to confirm ASBC receptor accessibility on probes and a sequential staining process to capture HIV gp140-specific B cells from human peripheral blood mononuclear cells. For complete details on the use and execution of this protocol, please refer to Townsley et al. (2021).
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Coloration et marquage
/
Lymphocytes B
/
Produits du gène env du virus de l'immunodéficience humaine
/
Cytométrie en flux
Limites:
Humans
Langue:
En
Journal:
STAR Protoc
Année:
2021
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique