Detection of Human Papillomavirus and p16INK4a Expression in Thai Patients with Oral Squamous Cell Carcinoma.
Head Neck Pathol
; 16(2): 444-452, 2022 Jun.
Article
de En
| MEDLINE
| ID: mdl-34590266
ABSTRACT
This study investigated the prevalence of human papillomavirus (HPV) infection in oral squamous cell carcinoma (OSCC) cases, as well as the association between HPV presence and p16INK4a expression, in Thai patients with OSCC. Eighty-one formalin-fixed paraffin-embedded specimens of OSCC were obtained. DNA extraction was performed; this was followed by nested polymerase chain reaction analysis to determine HPV DNA status, using consensus primers for the L1 region of HPV. HPV subtypes were determined by DNA sequencing. HPV-positive specimens and HPV-negative specimens from age- and sex-matched patients were subjected to immunohistochemical analysis to determine p16INK4a expression status. Of the 81 OSCC specimens, eight (9.9%) exhibited HPV DNA; DNA sequencing confirmed that the viral subtype was HPV-18 in all eight specimens. These eight HPV-positive specimens, as well as eight HPV-negative specimens from age- and sex-matched patients, were subjected to immunohistochemical analysis to determine p16INK4a expression status. Three of eight (37.8%) HPV-positive specimens and three of eight (37.8%) HPV-negative specimens showed positive p16INK4a expression findings. However, we did not find a significant association between HPV status and p16INK4a expression status in our OSCC samples. In conclusion, the prevalence of high-risk HPV was low in this group of OSCC patients; no association between HPV status and p16INK4a expression status was identified.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs de la bouche
/
Inhibiteur p16 de kinase cycline-dépendante
/
Infections à papillomavirus
/
Alphapapillomavirus
/
Carcinome épidermoïde de la tête et du cou
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limites:
Humans
Pays/Région comme sujet:
Asia
Langue:
En
Journal:
Head Neck Pathol
Sujet du journal:
NEOPLASIAS
/
PATOLOGIA
Année:
2022
Type de document:
Article
Pays d'affiliation:
Thaïlande