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Phylogenetically diverse bacteria isolated from tattoo inks, an azo dye-rich environment, decolorize a wide range of azo dyes.
Nho, Seong Won; Cui, Xuewen; Kweon, Ohgew; Jin, Jinshan; Chen, Huizhong; Moon, Mi Sun; Kim, Seong-Jae; Cerniglia, Carl E.
Affiliation
  • Nho SW; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
  • Cui X; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
  • Kweon O; Present address: Sichuan Institute for Food and Drug Control, Chengdu 611731, Sichuan, China.
  • Jin J; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
  • Chen H; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
  • Moon MS; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
  • Kim SJ; Office of Cosmetics and Colors, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, USA.
  • Cerniglia CE; Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, USA.
Ann Microbiol ; 71(1)2021 Sep 21.
Article de En | MEDLINE | ID: mdl-34744534
PURPOSE: There has been an interest in the microbial azo dye degradation as an optional method for the treatment of azo dye-containing wastes. Tattoo ink is an extremely unique azo dye-rich environment, which have never been explored in terms of microorganisms capable of degrading azo dyes. Previously, we isolated 81 phylogenetically diverse bacteria, belonging to 18 genera and 52 species, contaminated in tattoo inks. In this study, we investigated if these bacteria, which can survive in the azo dye-rich environment, have an ability to degrade azo dyes. METHODS: We conducted a two-step azo dye degradation (or decolorization) assay. In step 1, a high-throughput degradability assay was done for 79 bacterial isolates using Methyl Red and Orange II. In step 2, a further degradation assay was done for 10 selected bacteria with a representative of 11 azo dyes, including 3 commercial tattoo ink azo dyes. Degradation of azo dyes were calculated from measuring optical absorbance of soluble dyes at specific wavelengths. RESULTS: The initial high-throughput azo dye assay (step 1) showed that 79 isolates had a complete or partial degradation of azo dyes; > 90% of Methyl Red and Orange II were degraded within 24 h, by 74 and 20 isolates, respectively. A further evaluation of azo dye degradability for 10 selected isolates in step 2 showed that the isolates, belonging to Bacillus, Brevibacillus, Paenibacillus, and Pseudomonas, exhibited an excellent decolorization ability for a wide range of azo dyes. CONCLUSIONS: This study showed that phylogenetically diverse bacteria, isolated from azo dye-rich tattoo inks, is able to degrade a diverse range of azo dyes, including 3 azo dyes used in commercial tattoo inks. Some of the strains would be good candidates for future studies to provide a systematic understanding of azo dye degradation mechanisms.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Ann Microbiol Année: 2021 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Italie

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Langue: En Journal: Ann Microbiol Année: 2021 Type de document: Article Pays d'affiliation: États-Unis d'Amérique Pays de publication: Italie