Allelic variation in class I HLA determines CD8+ T cell repertoire shape and cross-reactive memory responses to SARS-CoV-2.
Sci Immunol
; 7(67): eabk3070, 2022 01 21.
Article
de En
| MEDLINE
| ID: mdl-34793243
ABSTRACT
Effective presentation of antigens by human leukocyte antigen (HLA) class I molecules to CD8+ T cells is required for viral elimination and generation of long-term immunological memory. In this study, we applied a single-cell, multiomic technology to generate a unified ex vivo characterization of the CD8+ T cell response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) across four major HLA class I alleles. We found that HLA genotype conditions key features of epitope specificity, TCRα/ß sequence diversity, and the utilization of pre-existing SARS-CoV-2-reactive memory T cell pools. Single-cell transcriptomics revealed functionally diverse T cell phenotypes of SARS-CoV-2-reactive T cells, associated with both disease stage and epitope specificity. Our results show that HLA variations notably influence the CD8+ T cell repertoire shape and utilization of immune recall upon SARS-CoV-2 infection.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Antigènes d'histocompatibilité de classe I
/
Récepteur lymphocytaire T antigène, alpha-bêta
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Lymphocytes T CD8/
/
Allèles
/
SARS-CoV-2
/
COVID-19
/
Cellules T mémoire
Limites:
Humans
Langue:
En
Journal:
Sci Immunol
Année:
2022
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique