A dual cellular-heterogeneous catalyst strategy for the production of olefins from glucose.
Nat Chem
; 13(12): 1178-1185, 2021 12.
Article
de En
| MEDLINE
| ID: mdl-34811478
ABSTRACT
Living systems provide a promising approach to chemical synthesis, having been optimized by evolution to convert renewable carbon sources, such as glucose, into an enormous range of small molecules. However, a large number of synthetic structures can still be difficult to obtain solely from cells, such as unsubstituted hydrocarbons. In this work, we demonstrate the use of a dual cellular-heterogeneous catalytic strategy to produce olefins from glucose using a selective hydrolase to generate an activated intermediate that is readily deoxygenated. Using a new family of iterative thiolase enzymes, we genetically engineered a microbial strain that produces 4.3 ± 0.4 g l-1 of fatty acid from glucose with 86% captured as 3-hydroxyoctanoic and 3-hydroxydecanoic acids. This 3-hydroxy substituent serves as a leaving group that enables heterogeneous tandem decarboxylation-dehydration routes to olefinic products on Lewis acidic catalysts without the additional redox input required for enzymatic or chemical deoxygenation of simple fatty acids.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Alcènes
/
Acides gras
/
Glucose
Langue:
En
Journal:
Nat Chem
Sujet du journal:
QUIMICA
Année:
2021
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique