Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers.
STAR Protoc
; 2(4): 100939, 2021 12 17.
Article
de En
| MEDLINE
| ID: mdl-34825212
ABSTRACT
Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Marqueurs génétiques
/
Techniques génétiques
/
Mosaïcisme
Limites:
Animals
Langue:
En
Journal:
STAR Protoc
Année:
2021
Type de document:
Article
Pays d'affiliation:
Autriche