Distinct contributions of partial and full EMT to breast cancer malignancy.
Dev Cell
; 56(23): 3203-3221.e11, 2021 12 06.
Article
de En
| MEDLINE
| ID: mdl-34847378
ABSTRACT
Epithelial-mesenchymal transition (EMT) is a transient, reversible process of cell de-differentiation where cancer cells transit between various stages of an EMT continuum, including epithelial, partial EMT, and mesenchymal cell states. We have employed Tamoxifen-inducible dual recombinase lineage tracing systems combined with live imaging and 5-cell RNA sequencing to track cancer cells undergoing partial or full EMT in the MMTV-PyMT mouse model of metastatic breast cancer. In primary tumors, cancer cells infrequently undergo EMT and mostly transition between epithelial and partial EMT states but rarely reach full EMT. Cells undergoing partial EMT contribute to lung metastasis and chemoresistance, whereas full EMT cells mostly retain a mesenchymal phenotype and fail to colonize the lungs. However, full EMT cancer cells are enriched in recurrent tumors upon chemotherapy. Hence, cancer cells in various stages of the EMT continuum differentially contribute to hallmarks of breast cancer malignancy, such as tumor invasion, metastasis, and chemoresistance.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Tumeurs du sein
/
Marqueurs biologiques tumoraux
/
Régulation de l'expression des gènes tumoraux
/
Résistance aux médicaments antinéoplasiques
/
Transition épithélio-mésenchymateuse
/
Tumeurs du poumon
Limites:
Animals
/
Female
/
Humans
Langue:
En
Journal:
Dev Cell
Sujet du journal:
EMBRIOLOGIA
Année:
2021
Type de document:
Article
Pays d'affiliation:
Suisse