Cross-species opsonic activity of zebrafish fish-egg lectin on mouse macrophages.
Dev Comp Immunol
; 129: 104332, 2022 04.
Article
de En
| MEDLINE
| ID: mdl-34910945
ABSTRACT
Zebrafish Fish-egg lectin (zFEL) has been identified and proved to be a maternal factor with antibacterial and opsonic ability in fishes. In this study, we found that zFEL was capable of enhancing the phagocytosis of the bacteria by macrophages of mouse (RAW264.7 and mouse peritoneal macrophages), suggesting a cross-species function of zFEL in higher animals. Further studies showed that zFEL can active the antigen presentation ability by up-regulating the expression of CD80, CD86 and MHC II. Meanwhile, zFEL also promoted the polarization of macrophages to M1-type, which was confirmed by the increase of cytokines TNF-α and IL-6. The expression of p38 gene was up-regulated in macrophages preincubated with zFEL. Taken together, zFEL appears opsonic function in mammal macrophages and has potential application in immunomodulation.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Lectines
Type d'étude:
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
Dev Comp Immunol
Année:
2022
Type de document:
Article