Pidotimod increases inflammation in wounded zebrafish embryos.
Fish Shellfish Immunol
; 120: 429-433, 2022 Jan.
Article
de En
| MEDLINE
| ID: mdl-34922016
ABSTRACT
Pidotimod (PDT) is a synthetic dipeptide molecule which can improve immune responses in mice and humans, protecting hosts from infection. However, the exact mechanism of protection remains ill-defined. The effect of pidotimod has not yet been investigated in the inflammatory response of zebrafish. In this study, we used tail wound and infection models of zebrafish to study the effect of PDT on inflammation. We found that zebrafish larvae were sensitive to PDT immersion causing toxicity at doses above 50 µg/mL. The tail wound assay showed that PDT increased the recruitment of neutrophils and macrophages to the wound site and promoted the transcription of the pro-inflammatory cytokine il1b. However, we did not observe protection of uropathogenic Escherichia coli or Mycobacterium marinum infected zebrafish larvae following PDT treatment. This study provides a new platform for PDT research, which is worthy of further research to identify further effects of PDT therapy.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Acide pidolique
/
Danio zébré
/
Thiazolidines
/
Inflammation
Limites:
Animals
Langue:
En
Journal:
Fish Shellfish Immunol
Sujet du journal:
BIOLOGIA
/
MEDICINA VETERINARIA
Année:
2022
Type de document:
Article