Nanoribbon Biosensor in the Detection of miRNAs Associated with Colorectal Cancer.
Micromachines (Basel)
; 12(12)2021 Dec 18.
Article
de En
| MEDLINE
| ID: mdl-34945431
ABSTRACT
A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10-17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Type d'étude:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Langue:
En
Journal:
Micromachines (Basel)
Année:
2021
Type de document:
Article
Pays d'affiliation:
Russie