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Structures and therapeutic potential of anti-RBD human monoclonal antibodies against SARS-CoV-2.
Huang, Kuan-Ying A; Zhou, Daming; Tan, Tiong Kit; Chen, Charles; Duyvesteyn, Helen M E; Zhao, Yuguang; Ginn, Helen M; Qin, Ling; Rijal, Pramila; Schimanski, Lisa; Donat, Robert; Harding, Adam; Gilbert-Jaramillo, Javier; James, William; Tree, Julia A; Buttigieg, Karen; Carroll, Miles; Charlton, Sue; Lien, Chia-En; Lin, Meei-Yun; Chen, Cheng-Pin; Cheng, Shu-Hsing; Chen, Xiaorui; Lin, Tzou-Yien; Fry, Elizabeth E; Ren, Jingshan; Ma, Che; Townsend, Alain R; Stuart, David I.
Affiliation
  • Huang KA; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
  • Zhou D; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
  • Tan TK; Genomics Research Center, Academia Sinica, Taipei, Taiwan.
  • Chen C; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford, UK.
  • Duyvesteyn HME; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Zhao Y; Medigen Vaccine Biologics Corporation, Taipei, Taiwan.
  • Ginn HM; Temple University, Philadelphia, PA 19122, USA.
  • Qin L; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford, UK.
  • Rijal P; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford, UK.
  • Schimanski L; Diamond Light Source Ltd, Harwell Science & Innovation Campus, Didcot, UK.
  • Donat R; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford, UK.
  • Harding A; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Gilbert-Jaramillo J; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • James W; MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK.
  • Tree JA; Sir William Dunn School of Pathology, South Park Road, University of Oxford, UK.
  • Buttigieg K; Sir William Dunn School of Pathology, South Park Road, University of Oxford, UK.
  • Carroll M; Sir William Dunn School of Pathology, South Park Road, University of Oxford, UK.
  • Charlton S; National Infection Service, Public Health England, Porton Down, Salisbury, UK.
  • Lien CE; National Infection Service, Public Health England, Porton Down, Salisbury, UK.
  • Lin MY; National Infection Service, Public Health England, Porton Down, Salisbury, UK.
  • Chen CP; National Infection Service, Public Health England, Porton Down, Salisbury, UK.
  • Cheng SH; Medigen Vaccine Biologics Corporation, Taipei, Taiwan.
  • Chen X; Institute of Public Health, National Yang-Ming Chiao Tung University, Taipei, Taiwan.
  • Lin TY; Medigen Vaccine Biologics Corporation, Taipei, Taiwan.
  • Fry EE; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, and National Yang-Ming University, Taipei, Taiwan.
  • Ren J; Department of Infectious Diseases, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, and Taipei Medical University, Taipei, Taiwan.
  • Ma C; Genomics Research Center, Academia Sinica, Taipei, Taiwan.
  • Townsend AR; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
  • Stuart DI; Division of Structural Biology, Nuffield Department of Medicine, University of Oxford, The Wellcome Centre for Human Genetics, Headington, Oxford, UK.
Theranostics ; 12(1): 1-17, 2022.
Article de En | MEDLINE | ID: mdl-34987630
ABSTRACT

Background:

Administration of potent anti-receptor-binding domain (RBD) monoclonal antibodies has been shown to curtail viral shedding and reduce hospitalization in patients with SARS-CoV-2 infection. However, the structure-function analysis of potent human anti-RBD monoclonal antibodies and its links to the formulation of antibody cocktails remains largely elusive.

Methods:

Previously, we isolated a panel of neutralizing anti-RBD monoclonal antibodies from convalescent patients and showed their neutralization efficacy in vitro. Here, we elucidate the mechanism of action of antibodies and dissect antibodies at the epitope level, which leads to a formation of a potent antibody cocktail.

Results:

We found that representative antibodies which target non-overlapping epitopes are effective against wild type virus and recently emerging variants of concern, whilst being encoded by antibody genes with few somatic mutations. Neutralization is associated with the inhibition of binding of viral RBD to ACE2 and possibly of the subsequent fusion process. Structural analysis of representative antibodies, by cryo-electron microscopy and crystallography, reveals that they have some unique aspects that are of potential value while sharing some features in common with previously reported neutralizing monoclonal antibodies. For instance, one has a common VH 3-53 public variable region yet is unusually resilient to mutation at residue 501 of the RBD. We evaluate the in vivo efficacy of an antibody cocktail consisting of two potent non-competing anti-RBD antibodies in a Syrian hamster model. We demonstrate that the cocktail prevents weight loss, reduces lung viral load and attenuates pulmonary inflammation in hamsters in both prophylactic and therapeutic settings. Although neutralization of one of these antibodies is abrogated by the mutations of variant B.1.351, it is also possible to produce a bi-valent cocktail of antibodies both of which are resilient to variants B.1.1.7, B.1.351 and B.1.617.2.

Conclusions:

These findings support the up-to-date and rational design of an anti-RBD antibody cocktail as a therapeutic candidate against COVID-19.
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Mots clés

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: SARS-CoV-2 / Traitements médicamenteux de la COVID-19 / Anticorps monoclonaux Type d'étude: Prognostic_studies Limites: Animals / Female / Humans Langue: En Journal: Theranostics Année: 2022 Type de document: Article Pays d'affiliation: Taïwan

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: SARS-CoV-2 / Traitements médicamenteux de la COVID-19 / Anticorps monoclonaux Type d'étude: Prognostic_studies Limites: Animals / Female / Humans Langue: En Journal: Theranostics Année: 2022 Type de document: Article Pays d'affiliation: Taïwan
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