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The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells.
Melani, Rafael D; Gerbasi, Vincent R; Anderson, Lissa C; Sikora, Jacek W; Toby, Timothy K; Hutton, Josiah E; Butcher, David S; Negrão, Fernanda; Seckler, Henrique S; Srzentic, Kristina; Fornelli, Luca; Camarillo, Jeannie M; LeDuc, Richard D; Cesnik, Anthony J; Lundberg, Emma; Greer, Joseph B; Fellers, Ryan T; Robey, Matthew T; DeHart, Caroline J; Forte, Eleonora; Hendrickson, Christopher L; Abbatiello, Susan E; Thomas, Paul M; Kokaji, Andy I; Levitsky, Josh; Kelleher, Neil L.
Affiliation
  • Melani RD; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Gerbasi VR; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Anderson LC; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA.
  • Sikora JW; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Toby TK; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Hutton JE; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Butcher DS; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA.
  • Negrão F; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Seckler HS; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Srzentic K; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Fornelli L; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Camarillo JM; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • LeDuc RD; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Cesnik AJ; Department of Genetics, Stanford University, Stanford, CA, USA.
  • Lundberg E; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden.
  • Greer JB; Department of Genetics, Stanford University, Stanford, CA, USA.
  • Fellers RT; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden.
  • Robey MT; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • DeHart CJ; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Forte E; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Hendrickson CL; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
  • Abbatiello SE; Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
  • Thomas PM; Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
  • Kokaji AI; National High Magnetic Field Laboratory, Florida State University, Tallahassee, FL, USA.
  • Levitsky J; Thermo Fisher Scientific, Cambridge, MA, USA.
  • Kelleher NL; Department of Molecular Biosciences, Department of Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, USA.
Science ; 375(6579): 411-418, 2022 01 28.
Article de En | MEDLINE | ID: mdl-35084980
ABSTRACT
Human biology is tightly linked to proteins, yet most measurements do not precisely determine alternatively spliced sequences or posttranslational modifications. Here, we present the primary structures of ~30,000 unique proteoforms, nearly 10 times more than in previous studies, expressed from 1690 human genes across 21 cell types and plasma from human blood and bone marrow. The results, compiled in the Blood Proteoform Atlas (BPA), indicate that proteoforms better describe protein-level biology and are more specific indicators of differentiation than their corresponding proteins, which are more broadly expressed across cell types. We demonstrate the potential for clinical application, by interrogating the BPA in the context of liver transplantation and identifying cell and proteoform signatures that distinguish normal graft function from acute rejection and other causes of graft dysfunction.
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Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Cellules sanguines / Cellules de la moelle osseuse / Protéines du sang / Isoformes de protéines / Protéome / Bases de données de protéines Limites: Humans Langue: En Journal: Science Année: 2022 Type de document: Article Pays d'affiliation: États-Unis d'Amérique
Texte intégral
Ajouter à My VHL
Imprimer
XML
PubMed Links
Recherche sur Google

Texte intégral: 1 Collection: 01-internacional Base de données: MEDLINE Sujet principal: Cellules sanguines / Cellules de la moelle osseuse / Protéines du sang / Isoformes de protéines / Protéome / Bases de données de protéines Limites: Humans Langue: En Journal: Science Année: 2022 Type de document: Article Pays d'affiliation: États-Unis d'Amérique