Noradrenaline increases intracellular Ca2+ concentration in epithelial cells via α2-adrenoceptors in isolated mouse ileal crypts.
J Pharmacol Sci
; 148(3): 337-341, 2022 Mar.
Article
de En
| MEDLINE
| ID: mdl-35177214
ABSTRACT
The stimulation of α2-adrenoceptors caused a transient increase of intracellular calcium concentration ([Ca2+]i) monitored by ratiometry using Fura-2 in epithelial cells including enterochromaffin cells in isolated mouse ileal crypts, while stimulation of α1-and ß-adrenoceptors had no effect. The effect of noradrenaline was suppressed by α2-adrenoceptor antagonists, but not by α1-and ß-adrenoceptor antagonists, and partially suppressed by Ni2+ and nicardipine, but not by ω-conotoxin and ω-agatoxin. These results suggest that noradrenaline causes an increase of [Ca2+]i by the influx of extracellular Ca2+ through certain Ca2+ channels via α2-adrenoceptors in epithelial cells of mouse ileal crypts.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Norépinéphrine
/
Calcium
/
Récepteurs alpha-2 adrénergiques
/
Cellules épithéliales
/
Iléum
Limites:
Animals
Langue:
En
Journal:
J Pharmacol Sci
Sujet du journal:
FARMACOLOGIA
Année:
2022
Type de document:
Article
Pays d'affiliation:
Japon