Ratiometric fluorimetric and colorimetric probe for sensing and imaging pH changes in living cells.
Luminescence
; 37(5): 791-795, 2022 May.
Article
de En
| MEDLINE
| ID: mdl-35274451
Cell, enzyme, and tissue activity in living organisms are closely related to intracellular pH. Detecting the changes of intracellular pH is important to understanding the physiological and pathological changes in the process of crucial cell metabolism. A pH probe (HTBI) based on hemicyanine was synthesized. The probe solution displayed a marked colour change from yellow to amaranth with the pH increase from neutral to basic; simultaneously, the emission spectra showed a significant red shift. The probe exhibited a ratiometric fluorescence emission (F586nm /F542nm ) characteristic of pKa 8.82. As expected, HTBI exhibited high sensitivity and selectivity for pH, fine photostability, reversibility, and low cytotoxicity. Therefore, it would be a very useful tool for measuring the intracellular pH changes.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Colorimétrie
/
Colorants fluorescents
Langue:
En
Journal:
Luminescence
Sujet du journal:
BIOFISICA
/
BIOQUIMICA
Année:
2022
Type de document:
Article
Pays d'affiliation:
Chine
Pays de publication:
Royaume-Uni