Cytochrome P450 26A1 regulates the clusters and killing activity of NK cells during the peri-implantation period.
J Cell Mol Med
; 26(8): 2438-2450, 2022 04.
Article
de En
| MEDLINE
| ID: mdl-35297206
ABSTRACT
Cytochrome P450 26A1 (CYP26A1) plays a vital role in early pregnancy in mice. Our previous studies have found that CYP26A1 affects embryo implantation by modulating natural killer (NK) cells, and that there is a novel population of CYP26A1+ NK cells in the uteri of pregnant mice. The aim of this study was to investigate the effects of CYP26A1 on the subsets and killing activity of NK cells. Through single-cell RNA sequencing (scRNA-seq), we identified four NK cell subsets in the uterus, namely, conventional NK (cNK), tissue-resident NK (trNK) 1 and 2, and proliferating trNK (trNKp). The two most variable subpopulations after uterine knockdown of CYP26A1 were trNKp and trNK2 cells. CYP26A1 knockdown significantly downregulated the expression of the NK cell function-related genes Cd44, Cd160, Vegfc, and Slamf6 in trNK2 cells, and Klra17 and Ogn in trNKp cells. Both RNA-seq and cytotoxicity assays confirmed that CYP26A1+ NK cells had low cytotoxicity. These results indicate that CYP26A1 may affect the immune microenvironment at the maternal-foetal interface by regulating the activity of NK cells.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Implantation embryonnaire
/
Cellules tueuses naturelles
Type d'étude:
Prognostic_studies
Limites:
Animals
/
Pregnancy
Langue:
En
Journal:
J Cell Mol Med
Sujet du journal:
BIOLOGIA MOLECULAR
Année:
2022
Type de document:
Article
Pays d'affiliation:
Chine