[Transcriptome analysis on responses of leaf photosynthesis and nitrogen metabolism of Larix gmelinii to environmental change]. / å ´å®‰è½å¶æ¾å¶å ‰åˆä¸Žæ°®ä»£è°¢å¯¹çŽ¯å¢ƒå˜åŒ–å“åº”çš„è½¬å½•ç»„åˆ†æž.

ABSTRACT

To reveal molecular mechanisms underlying photosynthesis responses of Dahurian larch (Larix gmelinii) to environmental changes, we used the high-throughput sequencing technology to sequence the transcriptome of larch leaves from four latitudinal sites with different environmental conditions, and compared differential expression genes (DEGs). The four sites from high- to low-latitude were Tahe (52°52' N), Songling (50°72' N), Heihe (49°22' N), and Dailing (47°08' N). A total of 282428811 clean reads were sequenced out, among which the abundace of DEGs were 16915, 18812, 28536, 20635, 29957 and 23617 for the Tahe-Songling, Tahe-Heihe, Tahe-Dailing, Songling-Heihe, Songling-Dailing, and Heihe-Dailing comparisons, respectively. The expression of nine Psb genes family (i.e., PsbB, PsbK, PsbO, PsbP, PsbQ, PsbS, PsbW, Psb27, and Psb28) encoding Photosystem Ⅱ and that of three genes (ATPF1A,atpA, ATPF1G, atpG, and ATPF1D, atpH) encoding F-type ATPase, which were involved in photosynthesis pathway, were significantly up-regulated with increasing environmental differences among the sites. A similar up-regulation pattern occurred for the expression of genes encoding glutamine synthetase (glnA, GLUL), nitrate reductase (NR), and carbonic anhydrase (cynT, can) that were involved in nitrogen metabolism pathway. The numbers of DEGs and up-regulated genes increased with the increases in environmental changes among the sites, resulting in inter-site divergence of photosynthetic capacity of larch trees.