Subchronic Graphene Exposure Reshapes Skin Cell Metabolism.
J Proteome Res
; 21(7): 1675-1685, 2022 07 01.
Article
de En
| MEDLINE
| ID: mdl-35611947
ABSTRACT
In recent years, the toxicity of graphene-related materials (GRMs) has been evaluated in diverse models to guarantee their safety. In most applications, sublethal doses of GRMs contact human barriers such as skin in a subchronic way. Herein, the subchronic effect (30 day exposure) of three GRMs (GO 1, GO 2, and FLG) with different oxidation degrees and sizes was studied. The effects of these materials on human skin cells, HaCaTs, were assayed through high-throughput metabolic-based readout and other cell-based assays. A differential effect was found between the different GRMs. GO 2 induced a metabolic remodeling in epithelial cells, increasing the level of tricarboxylic acid components, mirrored by increased cell proliferation and changes in cell phenotype. The oxidation degree, size, and method of manufacture of GRMs dictated harmful effects on cell metabolism and behavior generated by nontoxic exposures. Therefore, a "safe by design" procedure is necessary when working with these nanomaterials.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Nanostructures
/
Graphite
Langue:
En
Journal:
J Proteome Res
Sujet du journal:
BIOQUIMICA
Année:
2022
Type de document:
Article
Pays d'affiliation:
Espagne