Most mitochondrial dGTP is tightly bound to respiratory complex I through the NDUFA10 subunit.
Commun Biol
; 5(1): 620, 2022 06 23.
Article
de En
| MEDLINE
| ID: mdl-35739187
ABSTRACT
Imbalanced mitochondrial dNTP pools are known players in the pathogenesis of multiple human diseases. Here we show that, even under physiological conditions, dGTP is largely overrepresented among other dNTPs in mitochondria of mouse tissues and human cultured cells. In addition, a vast majority of mitochondrial dGTP is tightly bound to NDUFA10, an accessory subunit of complex I of the mitochondrial respiratory chain. NDUFA10 shares a deoxyribonucleoside kinase (dNK) domain with deoxyribonucleoside kinases in the nucleotide salvage pathway, though no specific function beyond stabilizing the complex I holoenzyme has been described for this subunit. We mutated the dNK domain of NDUFA10 in human HEK-293T cells while preserving complex I assembly and activity. The NDUFA10E160A/R161A shows reduced dGTP binding capacity in vitro and leads to a 50% reduction in mitochondrial dGTP content, proving that most dGTP is directly bound to the dNK domain of NDUFA10. This interaction may represent a hitherto unknown mechanism regulating mitochondrial dNTP availability and linking oxidative metabolism to DNA maintenance.
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Complexe I de la chaîne respiratoire
/
Nucléotide désoxyguanylique
/
NADH dehydrogenase
Limites:
Humans
Langue:
En
Journal:
Commun Biol
Année:
2022
Type de document:
Article
Pays d'affiliation:
Espagne