Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.
Angew Chem Int Ed Engl
; 61(36): e202209785, 2022 09 05.
Article
de En
| MEDLINE
| ID: mdl-35819825
ABSTRACT
The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CySC59A variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Diterpènes
Langue:
En
Journal:
Angew Chem Int Ed Engl
Année:
2022
Type de document:
Article
Pays d'affiliation:
Chine