Genomic hybrid capture assay to detect Borrelia burgdorferi: an application to diagnose neuroborreliosis in horses.
J Vet Diagn Invest
; 34(5): 909-912, 2022 Sep.
Article
de En
| MEDLINE
| ID: mdl-35864735
ABSTRACT
Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for Borrelia burgdorferi in CSF and the low specificity of serumCSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the B. burgdorferi flagellin gene was negative. However, we enriched B. burgdorferi DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified B. burgdorferi in the CSF of the horse, confirming a diagnosis of neuroborreliosis.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Maladie de Lyme
/
Groupe Borrelia burgdorferi
/
Borrelia burgdorferi
/
Maladies des chevaux
/
Maladies du système nerveux
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
Limites:
Animals
Langue:
En
Journal:
J Vet Diagn Invest
Sujet du journal:
MEDICINA VETERINARIA
Année:
2022
Type de document:
Article
Pays d'affiliation:
États-Unis d'Amérique