Macrophage migration inhibitory factor takes part in the lumbar ligamentum flavum hypertrophy.
Mol Med Rep
; 26(3)2022 09.
Article
de En
| MEDLINE
| ID: mdl-35904178
ABSTRACT
The present study aimed to observe the content difference of macrophage migration inhibitory factor [MIF; novoprotein recombinant human MIF (n6his) (ch33)], TGFß1 and MMP13 in patients with and without ligamentum flavum (LF) hypertrophy and investigate the roles of MIF in LF hypertrophy. The concentration of MIF, TGFß1 and MMP13 in LF were detected by ELISA in a lumbar spinal stenosis (LSS) group and a lumbar disc herniation (LDH) group. Culture of primary LFs and identification were performed for the subsequent study. Cell treatments and cell proliferation assay by CCK8 was performed. Western blot and quantitative PCR analysis were used to detect the expression of TGFß1, MMP13, type I collagen (COL1) and type III collagen (COL3) and Src which were promoted by MIF. The concentration of MIF, TGFß1 and MMP13 were higher in the LSS group compared with the LDH group. Culture of primary LFs and identification were performed. Significant difference in LFs proliferation occurred with treatment by MIF at a concentration of 40 nM for 48 h (P<0.05). The gene and protein expression of TGFß1, MMP13, COL1, COL3 and Src were promoted by MIF (P<0.05). Proliferation of LFs was induced by MIF and MIFinduced proliferation of LFs was inhibited by PP1 (a Src inhibitor). MIF may promote the proliferation of LFs through the Src kinase signaling pathway and can promote extracellular matrix changes by its proinflammatory effect. MIF and its mediated inflammatory reaction are driving factors of LF hypertrophy.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Sténose du canal vertébral
/
Facteurs inhibiteurs de la migration des macrophages
/
Ligament jaune
/
Déplacement de disque intervertébral
Type d'étude:
Prognostic_studies
Limites:
Humans
Langue:
En
Journal:
Mol Med Rep
Année:
2022
Type de document:
Article