Binding of phenochalasin A, an inhibitor of lipid droplet formation in mouse macrophages, on G-actin.
Drug Discov Ther
; 16(4): 148-153, 2022 Sep 17.
Article
de En
| MEDLINE
| ID: mdl-36002309
ABSTRACT
Phenochalasin A, a unique phenol-containing cytochalasin produced by the marine-derived fungus Phomopsis sp. FT-0211, was originally discovered in a cell morphological assay of observing the inhibition of lipid droplet formation in mouse peritoneal macrophages. To investigate the mode of action and binding proteins, phenochalasin A was radio-labeled by 125I. Iodinated phenochalasin A exhibited the same biological activity as phenochalasin A. [125I]Phenochalasin A was found to be associated with an approximately 40 kDa protein, which was identified as G-actin. Furthermore, detail analyses of F-actin formation in Chinese hamster ovary cells (CHO-K1 cells) indicated that phenochalasin A (2 µM) caused elimination of F-actin formation on the apical site of the cells, suggesting that actin-oriented specific function(s) in cytoskeletal processes are affected by phenochalasin A.
Mots clés
Texte intégral:
1
Collection:
01-internacional
Base de données:
MEDLINE
Sujet principal:
Actines
/
Gouttelettes lipidiques
Limites:
Animals
Langue:
En
Journal:
Drug Discov Ther
Année:
2022
Type de document:
Article
Pays d'affiliation:
Japon